UTIs are the most common bacterial infection and major cause of morbidity. The elderly are particularly susceptible with direct consequences including urosepsis and increased fall risk. The conventional urine culture suffers from suboptimal sensitivity and lengthy test time (3-5 days). In contrast, the new nucleic acid amplification-based assay (quantitative PCR), offers urinary tract pathogen identification and antibiotic resistance gene detection within the next day with a sensitivity that is estimated three times higher than the bacterial culture. The timely provision of enhanced microbial detection and concomitant antibiotic sensitivity results help improve antimicrobial stewardship and clinical outcome.